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Image Search Results
Journal: PLoS ONE
Article Title: Divergent antioxidant capacity of human islet cell subsets: A potential cause of beta-cell vulnerability in diabetes and islet transplantation
doi: 10.1371/journal.pone.0196570
Figure Lengend Snippet: (A) Representative plot of islet with expression of three anti-oxidant enzymes. (B) The level of MFI of three anti-oxidant enzymes in in human pancreatic α and β-cells was quantitatively evaluated using LSC/iCys. α-cell expression of catalase and GPX was significantly higher than that of β-cells. On the other hand, the difference of SOD expression in α and β-cell subsets was not observed. These data confirm the scarce expression of antioxidant enzymes in β-cells, which can contribute to their high susceptibility to ROS-mediated stress(N = 5).
Article Snippet: Samples were analyzed with assistance of the DRI Imaging Core using
Techniques: Expressing
Journal: PLoS ONE
Article Title: Divergent antioxidant capacity of human islet cell subsets: A potential cause of beta-cell vulnerability in diabetes and islet transplantation
doi: 10.1371/journal.pone.0196570
Figure Lengend Snippet: (A) Human islets were incubated with Hydrogen Peroxide (H 2 O 2 ) or Nitric oxide (NO) for 24 hour. Pancreatic β-cell or α-cell specific necrosis was assessed using Flow cytometry. Although there was no significant difference in the viability of α-cells (7AAD negative population in glucagon positive population), β-cell viability significantly decreased. Reduction in viability was calculated as the difference of viability in the specific population in untreated control islets versus treated islets for each islet preparation. (B) The percentage reduction of viability in β-cell was significantly higher than that of α-cells. (C) The cellular composition in human islets after oxidative stress was assessed using LSC/iCys. The β-cell/α-cell ratio in islet cells after oxidative stress was significantly decreased(N = 5).
Article Snippet: Samples were analyzed with assistance of the DRI Imaging Core using
Techniques: Incubation, Flow Cytometry, Control
Journal: PLoS ONE
Article Title: Divergent antioxidant capacity of human islet cell subsets: A potential cause of beta-cell vulnerability in diabetes and islet transplantation
doi: 10.1371/journal.pone.0196570
Figure Lengend Snippet: (A) Representative photograph of α- and β-cell with or without expression of 8OHdG after giving oxidative Stress. 8OHdG expression in β- or α-cells was assessed using LSC/iCys. (B) The average MFI level of 8OHdG in α-or β- cell subset after exposure to H 2 O 2 were calculated and compared to the vehicle (non-stimulated group). The amount of 8OHdG after oxidative stress significantly increased in β-cell, when compared to α-cell (N = 5).
Article Snippet: Samples were analyzed with assistance of the DRI Imaging Core using
Techniques: Expressing